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(C)an(n)alyze: determination of 16 cannabinoids inside flowers, oils and seeds


Research within cannabinoids for pharmaceutical purposes increases, likewise does the number of substances to be tested. Therefore, compared to the German pharmacopoeia (Deutsches Arzneibuch; DAB), an increase of 10 cannabinoids was performed for qualification and quantification with this work. The aim of the method development was to decrease the runtime and optimize the gradient program in comparison to the DAB method1. To ensure accuracy of the method, a validation was performed according to ICH Guidelines Q2 R12. Parameters for the validation were selectivity, linearity, repeatability and the recovery rate. The given specification was derived from the Association of Official Analytical Chemists (AOAC) with the given standard method performance requirements for cannabis flowers and oils3.

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