HPLC Method to Differentiate Four THC Stereoisomers Formed from Δ9-THC Degradation: (6aR,9R)-Δ10-THC, (6aR,9S)-Δ10-THC, 9(R)-Δ6a,10a-THC, and 9(S)-Δ6a,10a-THC
Jeffrey B. Williams, MS, Kathleen B. Calati, Kirk W. Hering, Ph.D., Roxanne E. Franckowski, MS, Weston J. Umstead, Ph.D., and Donna M. Iula, Ph.D.
contributed by Cayman Chemical |
- Δ10-THC and Δ6a,10a-THC isomers produced from Δ9-THC degradation have stereochemical similarities that are challenging to differentiate.
- Traditional reversed-phase (C18) HPLC analysis is not suitable for accurate determination of these isomers.
- A method using a chiral HPLC column under normal-phase conditions offers reliable and robust identification of the four stereoisomers.
- Use of a chiral HPLC stationary phase for characterization of these phytocannabinoid degradants is an essential tool for the determination of potency and safety.
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