HPLC Method to Differentiate Four THC Stereoisomers Formed from Δ9-THC Degradation: (6aR,9R)-Δ10-THC, (6aR,9S)-Δ10-THC, 9(R)-Δ6a,10a-THC, and 9(S)-Δ6a,10a-THC

Jeffrey B. Williams, MS, Kathleen B. Calati, Kirk W. Hering, Ph.D., Roxanne E. Franckowski, MS, Weston J. Umstead, Ph.D., and Donna M. Iula, Ph.D.

contributed by Cayman Chemical | 04/26/2021

Δ¹⁰-THC and Δ^6a,10a-THC isomers produced from Δ⁹-THC degradation have stereochemical similarities that are challenging to differentiate.
Traditional reversed-phase (C18) HPLC analysis is not suitable for accurate determination of these isomers.
A method using a chiral HPLC column under normal-phase conditions offers reliable and robust identification of the four stereoisomers.
Use of a chiral HPLC stationary phase for characterization of these phytocannabinoid degradants is an essential tool for the determination of potency and safety.

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