Sink or Swim
Could THC have an negative impact on sperm – and any subsequent embryos? Susan Murphy explains her research into cannabis and the sperm epigenome…
Joanna Cummings |
How did you investigate this?
We initially used a rapid urine screening test to measure 11-nor-9-carboxy-ΔTHC (THCCOOH; the primary metabolite of cannabis) and then verified the results using an enzyme immunoassay. Measurements for THC and other cannabinoids were performed using ultra performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) and by enzyme immunoassay (EIA). Our findings indicate that cannabis use (in humans) and THC exposure (in rats) are associated with changes in DNA methylation, meaning changes in the way genes are being regulated.
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