Cannabinoid Quantitation via Rugged and Adaptable HPLC/UHPLC Method Development
sponsored by Phenomenex
April 23, 2020
12pm EDT / 9am PDT / 5pm BST
Potency testing demands in the cannabis industry have exploded and are continuing to expand in product formulations, applications, and plant cultivations. Of the hundreds of known cannabinoid molecules, six have been of primary interest to quantitate (THC, THC-A, CBD, CBD-A, CBG, and CBG-A). With various regulations, such as the Farm Bill, distinguishing between marijuana and hemp through levels of intoxicating cannabinoids, specific and accurate quantitation of these cannabinoids is ever more critical and even more challenging, with a rising number and level of minor cannabinoids potentially present from chemical reactions or novel cultivars. Along with the sheer number of potentially interfering cannabinoids, their chromatographic profiles are a challenge to maintain as their retention profiles can have significant shifts from minute changes in common chromatographic conditions. Here we’ll cover the key underlying chromatographic factors to ensure robust and rugged potency method development that can be adapted readily to accommodate the dynamic landscape.
Learning Objectives of Webinar
- Mobile phase factors applied to method accuracy and ruggedness.
- Instrument and method transfer considerations in robustness.
- Critical cannabinoids resolved chromatographically.
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